آخرین محصولات
Establishment of a mouse model of inflammatory bowel disease using
Abstract and Figures. Background: Dextran sulfate sodium (DSS)-induced murine colitis is the most commonly used model for the analysis of the pathogenesis of inflammatory bowel disease (IBD) and ...
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CLINICAL USE OF A SYNTHETIC ANTI COAGULANT …
CLINICAL USE OF DEXTRAN SULFATE 111 This occurred also with heparin, and it might be suggested that the liver plays a great role in destruction or inactivation of anticoagulant sulfates. 00 1 60 J 50 i 2. 3 Hours 5 6 8 FIG. 3. Individual difference in clotting time response to 400 mg of dextran sulfate. Curve 1, 36·year·old , 55
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Colitis induced in mice with dextran sulfate sodium (DSS) is
In the acute dextran sodium sulfate (DSS) colitis model mice are fed with DSS polymers in the drinking water and this induces a colitis characterised by diarrhoea, bloody faeces, weight loss and a histological picture of inflammation and ulceration as seen in human IBD.2 As the acute inflammatory response is independent of T and B cells,3 the ...
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HDL Cholesterol Laboratory Procedure Manual
In this method a magnesium/dextran sulfate solution is first added to the specimen to form water-soluble complexes with non-HDL cholesterol fractions. These complexes are not reactive with the measuring reagents added in the second step. With addition of reagent 2, HDL-cholesterol esters are converted to HDL-cholesterol by PEG-cholesterol esterase.
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Purification or Removal of Viruses including Adeno-associated Virus
The dextran sulfate ligand (Figure 3.24) has affinity for several virus types, which makes Capto DeVirS suitable for different virus applications, for example vaccine manufacturing processes. The matrix of Capto DeVirS is based on highly cross-linked high-flow agarose that is highly rigid and offers outstanding pressure/flow properties ...
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In Vivo and In Vitro Study on the Efficacy of Terpinen-4-ol in Dextran
The purpose of this study was to investigate the protective effects of Terpinen-4-ol (TER) on dextran sulfate sodium (DSS)-induced experimental colitis and clarify the possible mechanisms. In vivo, an acute colitis model was used to confirm the anti-inflammatory activity and the possible mechanisms of TER in C57BL/6 and NLRP3−/− …
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Formulation and evaluation of injectable dextran sulfate
The dextran sulfate sodium (DSS) nanoparticles were formulated with gelation technique. The nanoparticles exhibited significant physicochemical and effective antibacterial properties, with zeta ...
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Dextran
Microsurgeons can use dextran to decrease the risk of vascular complications such as thrombosis. This mechanism occurs …
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Salt‐tolerant cation exchanger‐containing sulfate groups as a …
In summary, the sulfate ligand seems to be more hydrophobic and salt tolerant than the typical strong cation exchanger with immobilized sulfonium groups. As explained in the introduction, affinity resins carrying immobilized heparin and dextran sulfate groups were used for the purifications of clotting factors, especially FIX and …
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Polysaccharides from Sargassum fusiforme after UV/H2O2 …
In this study, degraded polysaccharides from Sargassum fusiforme (PSF-T2) were prepared by UV/H 2 O 2 treatment for 2 h, and its effects on ameliorating dextran sulfate sodium-induced colitis were evaluated using a mouse model. Results showed that PSF-T2 relieved colitis symptoms, characterized by increasing the colon length and body weight, …
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DEXTRAN SULFATE
The dextran sulfate magnesium chloride precipitation method using dextran sulfate with an approximate molecular weight of 50,000, optimized to minimize HDL loss and maximize apo B-containing lipoprotein precipitation, has been credentialed as an AACC Selected Method and is the most popular precipitation method in the US. Once precipitation is ...
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Quantitative determination of dextran sulfate and pentosan
In this work, we have measured the concentrations of dextran sulfate (DS) and pentosan polysulfate (PPS) using polyanion selective electrodes under chronopotentiometric method where the transition time was measured and controlled-current pulsed chronopotentiometric transductions, where the phase boundary potential (emf) …
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Dextran Sulfate Nanocarriers: Design, Strategies and Biomedical
1. Introduction. Dextran sulfate (DXS) is a hydrophilic, biodegradable, biocompatible and negatively charged polysaccharide. It is a highly branched polyanionic polysaccharide with a sulfur content of about 17%, which is approximately equivalent to ~2.3 sulfate groups per glucose unit (Figure 1).This sulfated polysaccharide is produced by …
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Dextran sulfate 5 in cell media and medicine
Dextran sulfate of low molecular weight – such as DS5 - is a widely used anti-coagulation agent 1 for cells and antibodies and has been used for this purpose by large providers of cell culture media for over a decade. DS5 …
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Dextran Sulfate Sodium-Induced Colitis
Dextran sulfate sodium (DSS)-induced colitis is a widely used experimental inflammation model to assess the functions of various molecules in the colon. By the following protocol, the extent of leukocyte infiltration in DSS-induced experimental colitis in WT and KO mice is examined. 1. Induce colitis in mice by adding 5% DSS (MW = 36,000 ...
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Two-color fluorescent
Effects of dextran sulfate in standard WISH. 24-hpf embryos were hybridized to a sim1a digoxigenin-labeled RNA probe, which was visualized by AP-BCIP/NBT chromogenic detection under identical conditions and staining times. Lateral views of embryonic brains (A,B) and trunks (C,D) are shown with anterior to the left. In (B,D) but …
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Impact of dextran sulfate in culture media on titration of vesicular
The results showed that the presence of dextran sulfate in the culture medium during production of virus interferes with VSV-GFP titration by plaque assay but not by TCID 50% assay. Dextran sulfate has less effects on titration by TCID 50% assay because the virus inoculum remains for a longer period of time with the cells using this …
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Validation of murine dextran sulfate sodium-induced colitis using …
Dextran sulfate sodium (DSS)-induced colitis is one of the most frequently used rodent models for inflammatory bowel disease (IBD). The aim of this study was to validate the murine DSS-induced colitis model using four therapeutic agents for IBD. C57BL/6 mice were exposed to 3% DSS for 5days followed by 7-9 days of water (acute inflammation) or ...
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Dextran Sulfate
Dextran sulfate sodium (DSS)-induced colitis is a widely used experimental inflammation model to assess the functions of various molecules in the colon. By the following protocol, the extent of leukocyte infiltration in DSS-induced experimental colitis in WT and KO mice is examined. 1. Induce colitis in mice by adding 5% DSS (MW = 36,000 ...
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Intracellular Delivery of β-Galactosidase Enzyme Using Arginase
Subsequently, seven alternate polymeric layers were made of poly-l-arginine (1 mg/mL in 0.5 M NaCl) and dextran sulfate (1 mg/mL in 0.5 M NaCl) to form eight layers with alternate negative and positive charges. Finally, removal of the calcium carbonate template was carried out by using 0.2 M EGTA (7.5 pH) solution.
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Preparation, characterization,
Fig. 2 shows the FT-IR spectra of curcumin loaded dextran sulphate–chitosan NPs (a) dextran sulphate (b), curcumin (c) and chitosan (d). In the spectrum of chitosan (d), a peak at 1648 cm −1 was observed and this corresponds to amide I bending vibration. The sulphate groups of dextran sulphate produces a number of …
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Silk fibroin nanoparticles enhance quercetin immunomodulatory
The aim of the present study was to evaluate the intestinal anti-inflammatory effects of quercetin when is administered loaded in silk fibroin nanoparticles (QSFN) in the dextran sulphate sodium experimental model of mouse colitis, which displays some similarities to human IBD.
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SAFETY DATA SHEET
Dextran Sulfate, Sodium Salt (M.W.: 40,000 - 50,000) Section 10. Stability and reactivity Hazardous decomposition products Conditions to avoid No specific data. Under normal conditions of storage and use, hazardous decomposition products should not be produced. Chemical stability The product is stable. No specific data.::: Incompatible materials :
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Dextran Sulfate
Dextran Sulfate. Dextran sulfate is a biocompatible highly branched polysaccharide of 1–6 and 1–4 glycosidic linkage, consisting of 2.3 sulfate groups (approx.) per glucosyl …
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Best hybridization buffer for DNA Hybridization? | ResearchGate
50% dextran sulfate 4 µl . Total 20 µl . Notes: Use H2O to adjust the total volume to 20 µl. Be sure that you are preparing the right amount of hybridization mixture instead of the number of ...
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Designing chitosan-dextran sulfate nanoparticles using charge …
The purpose of this study was to examine the effect of charge ratio on the formation and properties of the chitosan (CS)-dextran sulfate (DS) nanoparticles developed for the delivery of water-soluble small and large molecules, including proteins. Rhodamine 6G (R6G) and bovine serum albumin (BSA) were chosen as model molecules. CS-DS …
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Frontiers | Dietary Taxifolin Protects Against Dextran Sulfate …
Taxifolin is a natural antioxidant polyphenol with various bioactivities and has many beneficial effects on human gut health. However, little is known of its function on colitis. In this study, the protective effects of taxifolin on colitis symptoms, inflammation, signaling pathways, and colon microbiota were investigated using dextran sulfate …
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Dextran: Uses, Interactions, Mechanism of Action
875 rowsDextran is a low molecular weight dextran used as an adjunctive treatment …
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(PDF) MULTI-ORGAN METABOLIC PERTURBATION STUDY OF DEXTRAN SULFATE
This study was designed to investigate multiple organ toxicity of Dextran sulfate sodium-induced (DSS) ulcerative colitis using glycolytic and mitochondrial metabolizing enzymes as indices Twelve ...
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Dextran sulfate 5 in cell media and medicine
Dextran sulfate 5 is a widely-known anticoagulant. 2–4,4 However, some of the major players in the cell culture media do not always reveal the full ingredient list of their cell culture media although they can still use dextran sulfate in the formula. Companies selling cell culture media have often an extensive portfolio of media with ...
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